The aim of this study was to determine the extent and geographical pattern of intraspecific chemical and genetic variability of the lichen Hypogymnia physodes by comparing populations from different habitats. We analyzed the secondary lichen substances and their relative concentrations using HPTLC and HPLC in samples collected from sites with different environmental conditions. We identified seven lichen substances: the cortical atranorin and chloroatranorin, and the medullary physodalic, physodic, protocetraric, 3-hydroxyphysodic, and 2′-0-methylphysodic acids. The samples were uniform qualitatively, which means that H. physodes has only one chemotype. We detected quantitative chemical differences between the samples without any geographical pattern. We investigated 21 samples in order to study the connection between genotypic diversity of populations and geographical distribution. We determined the sequences of five loci (ITS, nucSSU, nucLSU, mitSSU, EF1α). We found no significant genetic differentiation among populations collected from different areas.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 48 • No. 6