Crossoglossa puipuiensis Damián & Mitidieri (Orchidaceae) is described and illustrated as a new species from Peru. It differs from C. steinii by having an orbicular (vs. ovate to ovate–elliptic) labellum bearing at its base two, erect, subquadrate to rectangular (vs. prostrate, lanceolate to ovate) calli. Crossoglossa neirynckiana and C. dalessandroi are reported as new to the Peruvian flora.