Twenty-eight polymorphic microsatellite markers for Japanese sea urchin (Strongylocentrotus intermedius) were developed from the EST database of a related species purple sea urchin (S. purpuratus). The characterization results showed the moderate polymorphisms at these loci. The number of alleles ranged from 2 to 5, and the values of Ho and Hc varied from 0.0667 to 0.9655 and 0.0655 to 0.7350, respectively. No significant linkage disequilibrium (LD) between pairs of loci was found and 21 loci conformed to the Hardy-Weinberg equilibrium (HWE). The results in this study well demonstrated the feasibility of microsatellite marker development via mining EST database of a genetically related species.
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Vol. 45 • No. 3