We conducted a nonlethal feeding experiment to measure diet/hair and diet/faeces trophic discrimination factors (TDFs) in two rodent species fed two diet types. The mean TDFs between diet and hair (expressed as δ¹³C/δ¹⁵N) were 2.14‰/3.44‰ for C3 plant-based fodder and 2.65‰/3.25‰ for fodder with 30% animal (insect) matter in yellow-necked mice (Apodemus flavicollis) and 1.66‰/4.67‰ for C3 plant-based fodder and 1.94‰/4.49‰ for fodder with 30% animal (insect) matter in bank voles (Myodes glareolus). As compared with the diet, faeces were depleted in ¹³C by about 0.75‰ and enriched in ¹⁵N by about 2‰. All bank voles and only half of yellow-necked mice had regrown hair at the end of the study (day 95). We analysed archived skins of those species and confirmed, that moult in bank voles is more intensive. Our results can be used for trophic studies of temperate-forest rodents and for meta-analyses on trophic discrimination.