In this paper, Macrobiotus naskreckii sp. nov., a new species of the hufelandi group from the Gorongosa National Park in Mozambique, is described. The analysis have revealed that M. naskreckii sp. nov. is most similar to M. kristenseni, M. ramoli and M. serratus. The new species differs from M. kristenseni mainly through a different shape of egg processes and clearly dentate lunules IV; from M. ramoli mainly by smaller eggs and a smaller number of egg processes on the circumference; from M. serratus mainly by the absence of the reticulation between the egg processes. This is a third record of tardigrades from Mozambique and apart of the new species reported here, three other taxa from Gorongosa National Park are provided: Minibiotus cf. intermedius, Minibiotus sp. and Paramacrobiotus cf. richtersi.