The new species Agraphorura xuae sp. nov. is described from continental Ecuador, the first onychiurid species record in this region. The new species is most similar to A. gambiarius (Murphy, 1965) from West Africa, but they can be separated easily by the number and length of p-chaetae between two inner posterior pso on the head, the number of chaetae on Th. I tergum, the presence of the male ventral organ and the ratio of the empodial appendage/unguis. A key to the world known species is provided. The list of all known species from continental Ecuador is presented.