The entire mitochondrial genome of the bristletail Pedetontus silvestrii (Mendes) (Archaeognatha: Machilidae) was sequenced. The genome is 15,879 bp and bears the standard set of genes common to all arthropods. Genome features like nucleotide composition and codon use are compared with three other bristletails. The gene trnQ was inverted compared with the order in other hexapods. The major noncoding region (the A T-rich region or control region) between the small ribosomal subunit (rrnS) and the trnI gene includes one extensive repeat region, which has two tandem repeats of a 128-bp sequence. A T content is higher than in other known species of Archaeognatha and Zygentoma but is similar to that in Collembola and Pterygota.