The association of insect-resistant plants with biological control is important in integrated management to reduce the density of pests below the economic injury level. The behavior, development, and predation of Podisus nigrispinus (Dallas, 1851) (Heteroptera: Pentatomidae) were studied with the prey Spodoptera frugiperda (J. E. Smith, 1797) fed on the leaves of the cotton cultivars ‘NuOpal’ (transgenic), ‘DeltaOpal,’ ‘FMX910,’ ‘FMX993,’ or ‘FMX996′ (conventional). The attraction of P. nigrispinus to S. frugiperda larvae fed on different cotton cultivars was similar. The predation of nymphs and adults, and the life cycle of the P. nigrispinus predator were higher for S. frugiperda larvae fed on the NuOpal; however, the total viability of P. nigrispinus was lower when predating on S. frugiperda fed the NuOpal. S. frugiperda acquires toxins from transgenic cotton, which can affect its natural predator P. nigrispinus.