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4 February 2016 Molecular Cloning and the Expression Profile of Vitellogenin in Relation to Tissue and Food Source in Apolygus lucorum (Hemiptera: Miridae)
Wenjing Li, Wei Yuan, Xincheng Zhao, Kongming Wu
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Abstract

Vitellogenin proteins (Vgs) are precursors of the major egg yolk proteins in many oviparous vertebrates and invertebrates. Here we cloned the full-length cDNA of the Vg gene of Apolygus lucorum, an omnivorous mirid bug that feeds on various agricultural crops and small insects. The gene has 6,131 base pairs (bp) with an open reading frame (ORF) of 5,988 bp, encoding a protein with 1,996 amino acids, with molecular mass 218.3 kDa and isoelectric point 9.02. Phylogenetic analysis showed that Vg gene has a high similarity with that in Hemiptera insects and was most closely related to the rice leaf bug, Trigonotylus caelestialium. The expression of Vg gene was significantly higher in the fat body than in other tissues in females (ovary, hemolymph, gut, and malpighian tubule). Vg gene expression was extremely low in newly emerged adult females and increased with age. The effect of different food sources on the expression level of Vg in adult females was investigated after they were fed on green bean pods (Gb), H. armigera eggs (He), or a combination of green bean pods and H. armigera eggs (GbHe). The results showed that transcript level of Vg gene in females was significantly higher on the GbHe diet than on Gb or He. Our results demonstrate that A. lucorum can acquire nutrients from both plant and animal origin food sources, and the combined food sources significantly facilitated Vg synthesis.

© The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Wenjing Li, Wei Yuan, Xincheng Zhao, and Kongming Wu "Molecular Cloning and the Expression Profile of Vitellogenin in Relation to Tissue and Food Source in Apolygus lucorum (Hemiptera: Miridae)," Annals of the Entomological Society of America 109(3), 350-356, (4 February 2016). https://doi.org/10.1093/aesa/saw005
Received: 14 September 2015; Accepted: 9 January 2016; Published: 4 February 2016
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KEYWORDS
Apolygus lucorum
mRNA expression
nutrition
vitellogenin
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