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27 July 2021 Assessment and Comparison of Two Different Methods to Extract Nucleic Acids From Individual Honey Bees
Rohan Swami, Brooke Ganser, David R. Tarpy, Micheline K. Strand, Hongmei Li-Byarlay
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Abstract

The honey bee is an excellent model system to study behavioral ecology, behavioral genetics, and sociogenomics. Nucleic acid-based analyses enable a broad scope of research in functional genomics, disease diagnostics, mutant screening, and genetic breeding. Multiple levels of analysis lead to a more comprehensive understanding of the causes of phenotypic variation by integrating genomic variation, transcriptomic profiles, and epigenomic information. One limitation, however, is the sample preparation procedures to obtain high quality DNA and RNA simultaneously, particularly from small amounts of material, such as tissues of individual bees. We demonstrate that it is feasible to perform dual extractions of DNA and RNA from a single individual bee and compare the quality and quantity of the extracted nucleic acids using two different types of methods. There was a greater total yield of DNA and RNA from ethanol-based extractions with minimal differences in overall concentration in ng/uL. We describe here the first validated method for dual extraction of DNA and RNA specifically from individual honey bees (Apis mellifera).

Published by Oxford University Press on behalf of Entomological Society of America 2021. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Rohan Swami, Brooke Ganser, David R. Tarpy, Micheline K. Strand, and Hongmei Li-Byarlay "Assessment and Comparison of Two Different Methods to Extract Nucleic Acids From Individual Honey Bees," Annals of the Entomological Society of America 114(5), 614-619, (27 July 2021). https://doi.org/10.1093/aesa/saab027
Received: 21 December 2020; Accepted: 14 June 2021; Published: 27 July 2021
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KEYWORDS
Apis
bee
Genetics
genomics
nucleic acid extraction
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