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12 May 2022 Four Insulin-Like Peptides Orchestrate Reproductive Signaling of the Green Lacewing, Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae)
Tingting Zhang, Xiaoping Liu, Lisheng Zhang, Mengqing Wang, Yuyan Li, Jianjun Mao
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Abstract

In insects, insulin signaling plays a pivotal role in the regulation of growth, metabolism, reproduction, and aging. Revealing the regulatory roles of insulin signaling in natural enemy insects is helpful for developing artificial approaches to promote reproduction. In this study, the expression of four insulin-like peptides (ILPs) in female adults of the natural predator Chrysopa pallens was analyzed by quantitative real-time-polymerase chain reaction (qRT-PCR) and their functions were investigated by RNA interference (RNAi). C. pallens ILP1 (CpILP1) and C. pallens ILP4 (CpILP4) showed a gradual increase in mRNA levels from early adult stages to later stages. However, C. pallens ILP2 (CpILP2) and C. pallens ILP3 (CpILP3) had an opposite expression curve with a high transcript level at the beginning of the adult stage and a gradual reduction thereafter. All four CpILPs showed high expression levels in the ovary, but CpILP2 transcripts were also abundant in fat bodies. Disruption of CpILP1 and CpILP2 expression by RNAi-mediated knockdown strikingly suppressed ovarian development and reduced C. pallens vitellogenin gene 1 (CpVg1) transcripts. Depletion of CpILP3 transcripts did not significantly decrease ovariole number but drastically reduced CpVg1 expression. Interference with the expression of CpILP4 significantly inhibited ovarian development but did not suppress CpVg1 expression. All gene knockdowns failed to dramatically impair total fecundity and egg quality.These data suggest that the four C. pallens ILPs are involved in reproductive signaling at different degrees.

© The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Tingting Zhang, Xiaoping Liu, Lisheng Zhang, Mengqing Wang, Yuyan Li, and Jianjun Mao "Four Insulin-Like Peptides Orchestrate Reproductive Signaling of the Green Lacewing, Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae)," Annals of the Entomological Society of America 115(4), 352-359, (12 May 2022). https://doi.org/10.1093/aesa/saac007
Received: 15 December 2021; Accepted: 5 April 2022; Published: 12 May 2022
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KEYWORDS
Chrysopa pallens
insulin signaling pathway
insulin-like peptide
ovary development
reproduction
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