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1 March 2000 Vitellogenin in Pupal Hemolymph of Diatraea grandiosella (Lepidoptera: Pyralidae)
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Abstract

Vitellogenin (Vg) concentration was determined in pupal hemolymph of female southwestern corn borer, Diatraea grandiosella (Dyar). Vitellogenin concentration increased significantly with pupal age. Vitellogenin level reached 7 mg/ml on day 7 after pupation. The vitellogenin was separable into 2 apoproteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with molecular weights of 145,000 for apoVg-I and 38,000 for apoVg-II. SDS-PAGE analysis revealed that vitellogenin polypetides in female pupal hemolymph appeared to be identical to those from eggs, but were absent in male and early female pupal hemolymph. A method to quantify vitellogenin by indirect ELISA is described. Results from this study and those of a previous study suggest that juvenile hormone does not have a role in vitellogenesis but does so in choriogenesis.

F. Zeng, S. Shu, S. B. Ramaswamy, and A. Srinivasan "Vitellogenin in Pupal Hemolymph of Diatraea grandiosella (Lepidoptera: Pyralidae)," Annals of the Entomological Society of America 93(2), 291-294, (1 March 2000). https://doi.org/10.1603/0013-8746(2000)093[0291:VIPHOD]2.0.CO;2
Received: 20 April 1999; Accepted: 1 August 1999; Published: 1 March 2000
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