Catalogue of the pseudoscorpions (Pseudoscorpiones) in František Miller's collection (Department of Zoology, National Museum, Prague)

Abstract. The present catalogue lists data for a total of 176 specimens belonging to 18 species in the pseudoscorpion collection of the Czech arachnologist František Miller (1902–1983), housed in the National Museum in Prague. The material was collected during 1940–1976 in the modern-day Czech Republic and Slovakia. For these two countries, especially noteworthy items are species such as Mesochelifer ressli, Rhacochelifer euboicus, Neobisium brevidigitatum and Neobisium cf. jugorum.


Professor
RNDr. František Miller, DrSc. (Fig. 1) was born in Kročehlavy near Kladno on 27 January 1902. After graduating at the Fac ulty of Science of the Charles University in Prague, he started to teach at secondary schools in the Slovak towns of Štubnianske (today Turčianske) Teplice (1929) and Žilina (1939), and in the Czech town of Jindřichův Hradec (1939). He became director of the sec ondary school in the small Czech town of Soběslav during the Second World War. In 1947, he obtained his habilitation at the University of Agriculture in Brno and worked there until his death on 14 January 1983 (Buchar 1997).
During his fruitful life (65 published papers), Miller pri marily studied spiders of the family Linyphiidae (Buchar 1997). As formalin pitfall traps and sieving belong to the most important collecting methods in arachnology, Miller's material also contains other soil or epigean invertebrates, in cluding pseudoscorpions. The majority of the material was collected in the surroundings of Miller's places of work. His large private collection was purchased by the National Muse um in Prague, Czech Republic, from Miller's widow, Jarmila Millerová, in 1983 and deposited in the Department of Zoo logy of this Museum under accession numbers 100/83 and 103/83 (e.g., Kůrka 1994, Dolejš & Kůrka 2013, Kocourek & Dolejš 2016. Beside spiders, the col lection also contained unsorted material of other invertebrates obtained together with spiders: harvestmen, pseudoscorpions, mites, centipedes, millipedes, isopods, etc. In this paper, we present a review of the pseudoscorpions (Pseudoscorpiones) found in the Miller's collection. It contains 176 specimens, re presenting 18 species in five families. The collection contains historical records of particular value for faunistic purposes (Krajčovičová et al. 2017).
The pseudoscorpion collection of the National Museum contains specimens preserved in ethanol, as well as some dry specimens. Most of the spirit material was collected by the former curator, Dr. Antonín Kůrka, from the Czech Republic and during inventory research in the newly established Brdy Protected Landscape Area ( Just et al. 2018). Further recent material was collected during expeditions of the Department of Entomology to the Dominican Republic, New Zealand, Puerto Rico and South Africa. The historical material (dry specimens and a few spirit specimens) comes from various destinations: besides the former Czechoslovakia (including the southwestern part of modern Ukraine), these include the Balkan Peninsula, Brazil, Italy and Mexico. Miller's collection is thus an important part of the pseudoscorpion collection of the National Museum.

Material and methods
All pseudoscorpion specimens are maintained in 80% etha nol. Almost all of them (with the exceptions of Rhacochelifer euboicus) were sexed and identified by the first author, using Christophoryová at al. (2011). Families are sorted systemati cally; genera and species are sorted alphabetically according to nomenclature used in Harvey (2013).
The data are arranged as follows: locality -(number of mapping grid square) -date of collection -number and sex of specimens -(inventory number).
The present administrative divisions of Europe are used. Within the Czech Republic, the historical regions of Bohe mia and Moravia are recognized following Kment (2009). The geographic position of localities ( Fig. 2) is given by grid squares after Buchar (1982) and, in the case of Czech settle ments, after Pruner & Míka (1996).
Images of selected specimens were made using an Olym pus SZX12 stereomicroscope equipped with an Olympus E510 or DP70 camera, and processed using the Quick PHOTO MICRO 2.3 (Promicra) software including the module Deep Focus 3.2. Abbreviations: D = deutonymph, P = protonymph, T = tritonymph.

Discussion
The material of pseudoscorpions from the collection of Prof. Miller forms a significant part of this order housed in the National Museum in Prague. Given that the main collecting methods used were formalin pitfall traps and sieving, it is not surprising that half of Miller's samples contain representatives of the family Neobisiidae, which are closely associated with the soil. This material includes the species Neobisium carcinoides, which is one of the most widespread European species (Harvey 2013) Christophoryová et al. 2012) and they seem to be typical for the leaf litter in Central Europe. The most interesting material of the genus Neobisium in the collection is that of the species N. brevidigitatum and N. cf. jugorum. Neobisium brevidigitatum was described from Romania (Beier 1928) and later recorded from Georgia, Poland and Slovakia (see Harvey 2013). Although detailed collecting information is lacking for the material from the High Tatra Mountains, it confirms the presence of this spe cies in the Western Carpathians, which was previously menti oned only from Great Fatra (Krumpál 1980) and, with doubt, from the Pienin Mountains (Rafalski 1967). The specimens of N. cf. jugorum from Modré pleso Lake in the High Tatra Mountains provide an additional record of this species from the Carpathians that was already recorded by Verner (1960) from these mountains. However, Miller's specimens from one locality show variability in the shape and size of the epistome from none in the female to sharp pronounced in some males (see Figs 710). All other characteristics correspond to the features typical to N. jugorum (e.g. Beier 1963). The fauna of the family Neobisiidae is still not well known from the Car pathian region and preliminary cytogenetic results indicate existence of additional taxa in this region (e.g. Šťáhlavský et al. 2012). The pronounced difference in the epistomes be tween males and females is not mentioned in this species and we cannot exclude the possibility that Miller's material repre sents in fact a new species with distinct sexual dimorphism. Miller collected several pseudoscorpion species a long time before the final published records for the Czech or Slo vak Republics. For example, his collection of Atemnus politus (Atemnidae) in 1956, close to Štúrovo, predates that of the female collected in 1974 in the same area that served to es tablish the presence of this species in Slovakia (Krumpálová & Krumpál 1993). Miller's specimens of Rhacochelifer euboicus (Cheliferidae) were also the first to be collected in Slovakia and his abundant material enabled the description of the va riability of morphological characteristics (Krajčovičová et al. 2017) from populations situated at the northern limit of its distribution (HernándezCorral et al. 2018). Among the rare species (in the Czech Republic and Slovakia) in the Miller's collection belongs also Mesochelifer ressli, a species usually found under the bark of the trees (e.g. Šťáhlavský & Chytil 2013).
It is evident that Miller's collection includes valuable material and provides important historical records for pseu doscorpions in the Czech Republic and Slovakia.