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20 August 2012 Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis
Juergen von Zum Hof, Karsten Schönrogge, James M. Cook, Michael G. Gardner
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Abstract

To date there have been only limited fine-scale investigations into the molecular ecology of the European hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers. We describe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97

© CSIRO 2012
Juergen von Zum Hof, Karsten Schönrogge, James M. Cook, and Michael G. Gardner "Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis," Australian Journal of Zoology 60(2), 108-110, (20 August 2012). https://doi.org/10.1071/ZO12042
Received: 18 April 2012; Accepted: 1 July 2012; Published: 20 August 2012
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KEYWORDS
genetic population structure
local adaptation
PCR
social parasite
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