Isolation and characterisation of polymorphic microsatellite loci for the neriid fly Telostylinus angusticollis using MiSeq sequencing

Anna M. Kopps; Russell Bonduriansky; Anthony S. Gilchrist; Angela J. Crean

doi:10.1071/ZO13017

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16 May 2013 Isolation and characterisation of polymorphic microsatellite loci for the neriid fly Telostylinus angusticollis using MiSeq sequencing

Anna M. Kopps, Russell Bonduriansky, Anthony S. Gilchrist, Angela J. Crean

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Australian J. of Zoology, 60(6):388-391 (2013). https://doi.org/10.1071/ZO13017

Abstract

The neriid fly Telostylinus angusticollis is being developed as a model organism for experimental research on developmental plasticity, nongenetic inheritance, ageing and sexual selection. No genetic information is currently available for T. angusticollis, so to facilitate paternity analyses for experimentation we characterised 17 polymorphic microsatellites for this species based on MiSeq sequences. The loci had, on average, 4.1 alleles and the observed heterozygosity ranged from 0.250 to 0.889. We show that MiSeq can be used successfully to develop microsatellite markers.

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Anna M. Kopps, Russell Bonduriansky, Anthony S. Gilchrist, and Angela J. Crean "Isolation and characterisation of polymorphic microsatellite loci for the neriid fly Telostylinus angusticollis using MiSeq sequencing," Australian Journal of Zoology 60(6), 388-391, (16 May 2013). https://doi.org/10.1071/ZO13017

Received: 11 February 2013; Accepted: 1 April 2013; Published: 16 May 2013

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