Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

Talat Hojat Ansari; Terry Bertozzi; Jessica Hacking; Steven J. B. Cooper; Michael G. Gardner

doi:10.1071/ZO14085

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17 February 2015 Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper, Michael G. Gardner

Author Affiliations +

Talat Hojat Ansari,¹ Terry Bertozzi,^2,3 Jessica Hacking,¹ Steven J. B. Cooper,^2,3 Michael G. Gardner^1,2,*

¹ASchool of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
²BEvolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.
³CAustralian Centre for Evolutionary Biology and Biodiversity and the School of Biological Sciences,

^*Corresponding author. Email: michael.gardner@flinders.edu.au

Australian J. of Zoology, 62(6):515-518 (2015). https://doi.org/10.1071/ZO14085

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Abstract

We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a ‘northern’ lineage (48 loci) and a ‘southern’ lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

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Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper, and Michael G. Gardner "Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species," Australian Journal of Zoology 62(6), 515-518, (17 February 2015). https://doi.org/10.1071/ZO14085

Received: 16 October 2014; Accepted: 1 February 2015; Published: 17 February 2015

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Australian Journal of Zoology
Vol. 62 • No. 6
March 2015

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Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper, Michael G. Gardner "Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species," Australian Journal of Zoology, 62(6), 515-518, (17 February 2015)

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