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17 February 2015 Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species
Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper, Michael G. Gardner
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Abstract

We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a ‘northern’ lineage (48 loci) and a ‘southern’ lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

© CSIRO 2014
Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper, and Michael G. Gardner "Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species," Australian Journal of Zoology 62(6), 515-518, (17 February 2015). https://doi.org/10.1071/ZO14085
Received: 16 October 2014; Accepted: 1 February 2015; Published: 17 February 2015
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KEYWORDS
DNA markers
PHYLOGEOGRAPHY
squamate.
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