The gall-forming fly family Fergusoninidae, in association with a mutualist nematode, induces galls on Myrtaceae. Traditionally, each fly species has been thought to be host-specific and targets a particular site on its host plant. One host species may be host to as many as four fly species, each with different oviposition sites, giving rise to a range of gall types. Third-instar fly larvae possess a distinctive sclerotised ‘dorsal shield’ of unknown function that varies morphologically across the genus. We use a phylogenetic approach to examine the relationship of the dorsal shield morphology to other elements of this complex system. A phylogeny of 41 species, estimated using Bayesian analysis of mtCOI sequences, indicated a strong correlation between dorsal shield morphology and the gall type associated with the larva. We discuss possible functions of the dorsal shield, and other factors that may have led to their phylogenetic distribution. In addition, we have identified cases where fly species have formed galls on more than one host species. In some instances it is possible that these associations are an opportunistic response to artificial tree plantings.

The endangered black-tailed dusky antechinus (Antechinus arktos) was described in 2014, so most aspects of its ecology are unknown. We examined diet composition and prey selection of A. arktos and a sympatric congener, the northern form of A. stuartii, at two sites in Springbrook National Park. Overall, taxa from 25 invertebrate orders were identified in the diets from 252 scat samples. Dietary components were similar for each species, but A. arktos consumed a higher frequency and volume of dipteran larvae and Diplopoda, while A. stuartii consumed more Coleoptera, Lepidoptera, Orthoptera and Isopoda. Both species of Antechinus had a higher percentage of ‘empty’ scats (devoid of any identifiable invertebrate material) in 2014 compared with 2015. The former was a drier year overall. Lower rainfall may have reduced abundance and diversity of arthropod prey, causing both species to supplement their diet with soft-bodied prey items such as earthworms, which are rarely detected in scats. Comparison of prey in scats with invertebrate captures from pitfall traps showed both species to be dietary generalists, despite exhibiting distinct preference and avoidance of certain prey categories. The ability of an endangered generalist marsupial to switch prey may be particularly advantageous considering the anticipated effects of climate change on Gondwanan rainforests during the mid-late 21st century.

This study was undertaken to determine the spawning season of the nurseryfish, a species with a unique method of parental care found only in northern Australia and southern New Guinea. Monthly samplings with an ichthyoplankton net at the same locality in the Adelaide River over multiple years yielded no larval nurseryfish in February, March, April and May. Larvae first appeared in June and were collected each month until January. From this we conclude that the nurseryfish spawning season in the Adelaide River is June–January. Twelve other larval fish species from 10 families were collected in the plankton tows with nurseryfish. Larval nurseryfish are readily identified and separated from other larval fishes by their peculiar rib anatomy, an important diagnostic character of Kurtus. The development of the unusual rib anatomy is examined with three-dimensional micro-computerised tomography scans of 12–21-mm standard length (SL) specimens. The bony protection of the swim bladder formed by the ribs is visible in the smallest postflexion specimens examined and is essentially complete by 19 mm SL. These ecological and anatomical observations add another pixel to the big picture of nurseryfish life history.

Marsupials and monotremes can be thought of as independent experiments in mammalian evolution. The discovery of the human male-determining gene, SRY, how it works, how it evolved and defined our sex chromosomes, well illustrates the value of comparing distantly related animals and the folly of relying on humans and mice for an understanding of the most fundamental aspects of mammalian biology. The 25th anniversary of the discovery of SRY seems a good time to review the contributions of Australian mammals to these discoveries.

The discovery of the mammalian sex determining gene, SRY, was a milestone in the history of human genetics. SRY opened up investigations into the pathway by which the genital ridge (bipotential gonad) becomes a testis. Studies of Australian mammals were important in the story of the discovery of SRY, not only in refuting the qualifications of the first candidate sex-determining gene, but also in confirming the ubiquity of SRY and raising questions as to how it works. Studies in marsupials also led to understanding of how SRY evolved from a gene on an autosome with functions in the brain and germ cells, and to identifying the ancestors of other genes on the human Y. The discovery that platypus have sex chromosomes homologous, not to the human XY, but to the bird ZW, dated the origin of the therian SRY and the XY chromosomes it defined. This led to important new models of how our sex chromosomes function, how they evolved, and what might befall this gene and the Y chromosome it defines.

The quokka (Setonyx brachyurus) is restricted to two offshore islands and small isolates on the mainland of south-western Australia. It displays a tendency to saltatorial locomotion and moves at speed by bipedal hopping, although it also uses its forelimbs at low speed. Its bipedal adaptation involves enlarged hind limbs, with elongated feet. The fibre type distribution of the elbow and knee extensors, and the ankle plantar flexors, in comparison with two eutherians, the quadrupedal rhesus monkey, as a locomotor generalist, and the jerboa, a small eutherian hopping species morphologically similar to the quokka, were studied. The quokka’s forelimb showed the same characteristics as that of the jerboa, lacking the fatigue-resistant Type I fibres that are used to sustain posture. As in the jerboa, the gastrocnemius lateralis was the muscle head with the highest proportion of fast twitch fibres. Muscular fibre pattern is not identical in the quokka and the jerboa hindlimb, but it appears that both species have similar anatomical adaptations to saltatorial locomotion. Differences in muscle fibre proportions could be due to several factors including, resting posture, body size and the propensity for elastic energy storage, the burrowing behaviour of the jerboa, but also to phylogenetic constraints where the adaptation to hop on the hindlimbs is a shared behaviour of the Macropodoidea (jerboas are the only Dipodidae to have elongated hindlimbs).

The dwarf galaxias (Galaxiella pusilla) and little galaxias (Galaxiella toourtkoourt) are both threatened freshwater fish from south-eastern Australia. Occasionally populations have been found with enlarged heads associated with the accumulation of ‘white balls’, but the cause of these deformities has not previously been investigated. In this study, histopathology and molecular techniques were employed to identify cysts extracted from the heads of Galaxiella species across six populations. Histopathology and DNA sequences from both mitochondrial (cytochrome c oxidase subunit I) and nuclear (ribosomal internal transcribed spacer 1) regions identified the cysts as metacercariae of Apatemon gracilis (Rudolphi, 1819), a cosmopolitan digenean trematode species. Heavy infestations of trematode metacercariae within Galaxiella populations are of concern due to the potential to cause increased mortality associated with altered behaviour of the fish host that increases the likelihood of predation. Direct mortality from infestations is also possible, but not quantified in this study.

Understanding patterns in the distribution and abundance of wildlife across the landscape can aid in identifying the relative importance of habitats for biodiversity conservation. We aimed to identify whether riparian habitats were more important than other areas in the landscape to small terrestrial vertebrates. The study site was surveyed using 450 pit traps distributed across riparian, midslope and ridge top habitat. Riparian sites had the greatest abundance of small vertebrates of the three habitats. During some months of the year, there was a significant difference in the composition of the faunal assemblage between habitats. Unsurprisingly, riparian habitats were particularly important for frog species and it was these species that accounted for the greater abundance in this habitat. Riparian habitat was less important for other taxonomic groups and the more floristically rich midslope and ridge habitats, which had a greater abundance of leaf litter, fallen logs and rock cover, were favoured by mammal and reptile species. The conservation of riparian sites, without the protection of other habitats, overlooks a substantial proportion of the biodiversity in the landscape. This study may help inform management decisions in the Upper Warren region and other similar forested landscapes, regarding the location and timing of fauna monitoring and the frequency of fuel reduction burns.