Translator Disclaimer
1 April 2003 A Longitudinal Study of a Novel Dot-Enzyme-Linked Immunosorbent Assay for Detection of Avian Influenza Virus
Author Affiliations +
Abstract

A monoclonal antibody (MAb)-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed that detected the epitopes specifically associated with avian influenza virus (AIV). The dot-ELISA detected the antigens of AIV directly from clinical and field specimens. Data obtained from experimentally AIV-infected specific-pathogen-free chickens and also the 2001/02 AIV outbreak of serotype H7N2 positive flocks in Pennsylvania indicated that the mean sensitivity (Se) of the dot-ELISA ranged between 45% and 68% and the mean specificity (Sp), between 85% and 90%. The values were derived from various clinical and field specimens when compared with virus isolation with embryonating chicken eggs. On routine AIV surveillance samples, the dot-ELISA achieved a 92%–100% Sp on the basis of testing over 1500 AIV surveillance samples that were confirmed negative by virus isolation. The dot-ELISA detected AIV antigens with a 5-µl allantoic fluid sample that contained a concentration of 0.4 hemagglutinating units. Furthermore, the dot-ELISA retained its specificity for AIV because no cross-reactions were obtained with various other avian viruses. The findings in this study indicated that the dot-ELISA was highly sensitive and specific and comparable with the commercial Directigen® test in the detection of AIV obtained from clinical and field specimens.

Huaguang Lu "A Longitudinal Study of a Novel Dot-Enzyme-Linked Immunosorbent Assay for Detection of Avian Influenza Virus," Avian Diseases 47(2), 361-369, (1 April 2003). https://doi.org/10.1637/0005-2086(2003)047[0361:ALSOAN]2.0.CO;2
Received: 2 October 2002; Published: 1 April 2003
JOURNAL ARTICLE
9 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top