ELISA Test for the Detection of Influenza H7 Antibodies in Avian Sera

G. Sala; P. Cordioli; A. Moreno-Martin; M. Tollis; E. Brocchi; A. Piccirillo; A. Lavazza

doi:10.1637/0005-2086-47.s3.1057

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G. Sala,¹ P. Cordioli,¹ A. Moreno-Martin,¹ M. Tollis,² E. Brocchi,¹ A. Piccirillo,¹ A. Lavazza¹

¹Istituto Zooprofilattico della Lombardia ed Emilia Romagna “B.Ubertini” Via A. Bianchi, 9-25124 Brescia, Italy
²Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy

Abstract

Using a monoclonal antibody (MAb) specific for the H7 influenza surface glycoproteins, a serological enzyme-linked immunosorbent assay (ELISA) test has been developed. This MAb was made using the low-pathogenicity (LP) avian influenza (AI) strain (BS2676/99) isolated in Italy during a recent outbreak. The test is able to detect H7 antibodies in avian sera. The H7 ELISA has a 99% concordance of results with the classical hemagglutination inhibition (HI) test.

Citation Download Citation

G. Sala, P. Cordioli, A. Moreno-Martin, M. Tollis, E. Brocchi, A. Piccirillo, and A. Lavazza "ELISA Test for the Detection of Influenza H7 Antibodies in Avian Sera," Avian Diseases 47(s3), 1057-1059, (1 September 2003). https://doi.org/10.1637/0005-2086-47.s3.1057

Received: 14 April 2002; Published: 1 September 2003

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