A NASBA Method to Detect High- and Low-Pathogenicity H5 Avian Influenza Viruses

R. A. Collins; L. S. Ko; K. L. So; T. Ellis; L. T. Lau; A. C. Hoi Yu

doi:10.1637/0005-2086-47.s3.1069

We can help you reset your password using the email address linked to your BioOne Complete account.

Registered users receive a variety of benefits including the ability to customize email alerts, create favorite journals list, and save searches. Please note that a BioOne web account does not automatically grant access to full-text content. An institutional or society member subscription is required to view non-Open Access content. Contact helpdesk@bioone.org with any questions.

All Fields are Required

First Name

Last/Family Name

Password

Password Requirements: Minimum 8 characters, must include as least one uppercase, one lowercase letter, and one number or permitted symbol Valid Symbols for password:
~ Tilde
! Exclamation Mark
@ At sign
$ Dollar sign
^ Caret
( Opening Parenthesis
) Closing Parenthesis
_ Underscore
. Period

Confirm Password

Web Account created successfully

R. A. Collins,¹ L. S. Ko,¹ K. L. So,² T. Ellis,³ L. T. Lau,¹ A. C. Hoi Yu^2,*

¹aHong Kong DNA Chips Ltd., 1805-6, 18/F, Lu Plaza, 2 Wing Yip Street, Kowloon, Hong Kong SAR, China
²bDepartment of Biology, The Hong Kong University of Science and Technology, Clear Water Bay Road, Ko
³cAgriculture, Fisheries, and Conservation Department, Castle Peak Veterinary Lab, San Fuk Road, Tuen

^*Corresponding author

Abstract

Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.

Citation Download Citation

R. A. Collins, L. S. Ko, K. L. So, T. Ellis, L. T. Lau, and A. C. Hoi Yu "A NASBA Method to Detect High- and Low-Pathogenicity H5 Avian Influenza Viruses," Avian Diseases 47(s3), 1069-1074, (1 September 2003). https://doi.org/10.1637/0005-2086-47.s3.1069

Received: 14 April 2002; Published: 1 September 2003

You currently do not have any folders to save your paper to! Create a new folder below.