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1 January 2004 Antigenic and Genomic Characterization of Turkey Enterovirus-Like Virus (North Carolina, 1988 Isolate): Identification of the Virus as Turkey Astrovirus 2
James S. Guy, Andrea M. Miles, Lynda Smith, Frederick J. Fuller, Stacy Schultz-Cherry
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Abstract

A small round virus (SRV) was isolated in 1988 from droppings of enteritis-affected turkeys in North Carolina and tentatively identified as an enterovirus on the basis of size (18–24 nm in diameter), intracytoplasmic morphogenesis, and a single-stranded RNA genome of approximately 7.5 kb. Additional characterization studies based on antigenic and genomic analyses were done to determine the relationship of this turkey enterovirus-like virus (TELV) to turkey astrovirus 2 (TAstV2), a recently characterized SRV of turkeys. Cross-immunofluorescence studies with TELV- and TAstV2-specific antisera indicated a close antigenic relationship between these viruses. TELV RNA was amplified by reverse transcriptase–polymerase chain reaction (RT-PCR) procedures with oligonucleotide primers specific for TAstV2 polymerase gene (open reading frame [ORF] 1b) and capsid protein gene (ORF 2). Subsequent sequence analyses of these TELV-derived RT-PCR products indicated a high degree of similarity with polymerase gene (98.8%) and capsid gene (96.9%) of TAstV2. These studies definitively identify TELV (North Carolina, 1988 isolate) as TAstV2.

James S. Guy, Andrea M. Miles, Lynda Smith, Frederick J. Fuller, and Stacy Schultz-Cherry "Antigenic and Genomic Characterization of Turkey Enterovirus-Like Virus (North Carolina, 1988 Isolate): Identification of the Virus as Turkey Astrovirus 2," Avian Diseases 48(1), 206-211, (1 January 2004). https://doi.org/10.1637/7077
Received: 9 June 2003; Published: 1 January 2004
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