Characterization of Monoclonal Antibodies Against Fowl Adenovirus Serotype 1 (FAV1) Isolated from Gizzard Erosion

Satoshi Taharaguchi; Hiroko Ito; Hideyuki Ohta; Kozo Takase

doi:10.1637/7460-102605R.1

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Satoshi Taharaguchi,¹ Hiroko Ito,¹ Hideyuki Ohta,² Kozo Takase^2,*

¹aLaboratory of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University, Kagoshima, Jap
²bThe Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan 860-8568

^*Corresponding author.

Abstract

Six clones of monoclonal antibodies (Mabs) to fowl adenovirus (FAV) serotype 1 were produced. All Mabs reacted positively by enzyme-linked immunosorbent assay. Three Mabs recognized the putative 100-kD hexon protein and reacted to serotype 1 specifically by western blot analysis but did not react to other FAV serotypes (2, 3, 4, 5, 6, 7, and 8a). These Mabs will be useful for immunodiagnosis of FAV serotype 1 infection in chickens with gizzard erosion and in further research studies involving the genomes and proteins of FAV serotype 1.

Citation Download Citation

Satoshi Taharaguchi, Hiroko Ito, Hideyuki Ohta, and Kozo Takase "Characterization of Monoclonal Antibodies Against Fowl Adenovirus Serotype 1 (FAV1) Isolated from Gizzard Erosion," Avian Diseases 50(3), 331-335, (1 September 2006). https://doi.org/10.1637/7460-102605R.1

Received: 26 October 2005; Accepted: 1 February 2006; Published: 1 September 2006

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