BioOne.org will be down briefly for maintenance on 17 December 2024 between 18:00-22:00 Pacific Time US. We apologize for any inconvenience.
How to translate text using browser tools
1 December 2006 A Comparative Study of Detecting Chlamydophila psittaci in Pet Birds Using Isolation in Embryonated Egg and Polymerase Chain Reaction
Beliz Saracel Çelebi, Seyyal Ak
Author Affiliations +
Abstract

This study, for the first time in Turkey, investigated the existence of Chlamydophila psittaci and determined the prevalence of its disease, chlamydiosis, in pet birds. Polymerase chain reaction (PCR) was compared with other testing methods that have been typically used in the diagnosis of C. psittaci. Fecal specimens (n = 96) of avian origin were tested by PCR and two identification methods, modified Gimenez staining (mGS) and direct fluorescein–conjugated monoclonal antibody staining (FA). The identification methods were implemented by staining the yolk sacs of embryonated chicken eggs inoculated at 6 days of age and harvested between 3 and 10 days after inoculation. Fecal specimens from pet birds were randomly collected from pet shops and homes. These specimens were then used to isolate C. psittaci and to detect its specific DNA. The inocula that were prepared from fecal specimens were then inoculated into yolks of 6-day-old embryonated chicken eggs. The preparations from egg yolk sacs were examined with mGS and direct FA after three blind passages. The PCR method was used to detect specific DNA in feces. In 96 fecal specimens, 33 (34.4%) were positive with PCR, 21 (21.9%) were positive with mGS, and 29 (30.2%) were positive with FA. Among 33 positive specimens with PCR, 28 specimens were positive with FA, and 20 specimens were positive with mGS. The sensitivity and specificity were 59% and 94% between FA and mGS, and 97% and 93% between FA and PCR, respectively.

Beliz Saracel Çelebi and Seyyal Ak "A Comparative Study of Detecting Chlamydophila psittaci in Pet Birds Using Isolation in Embryonated Egg and Polymerase Chain Reaction," Avian Diseases 50(4), 489-493, (1 December 2006). https://doi.org/10.1637/7518-021406R.1
Received: 13 February 2006; Accepted: 1 June 2006; Published: 1 December 2006
KEYWORDS
Chlamydophila psittaci
direct fluorescein staining
modified Gimenez staining
pet birds
polymerase chain reaction
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top