Translator Disclaimer
1 June 2007 The Mycoplasma gallisepticum 16S–23S rRNA Intergenic Spacer Region Sequence as a Novel Tool for Epizootiological Studies
Author Affiliations +
Abstract
Mycoplasma gallisepticum (MG) contains two sets of rRNA genes (5S, 16S and 23S) in its genome, but only one of the two is organized in an operon cluster and contains a unique 660-nucleotide intergenic spacer region (IGSR) between the 16S and the 23S rRNA genes. We designed a polymerase chain reaction (PCR) for the specific amplification of the complete MG IGSR segment. The MG IGSR PCR was tested on 18 avian mollicute species and was confirmed as MG specific. The reaction sensitivity was demonstrated by comparing it to the well-established MG mgc2 PCR. The MG IGSR sequence was found to be highly variable (discrimination [D] index of 0.950) among a variety of MG laboratory strains, vaccine strains, and field isolates. The sequencing of the MG IGSR appears to be a valuable single-locus sequence typing (SLST) tool for MG isolate differentiation in diagnostic cases and epizootiological studies.
Ziv Raviv, S. Callison, N. Ferguson-Noel, V. Laibinis, R. Wooten and S. H. Kleven "The Mycoplasma gallisepticum 16S–23S rRNA Intergenic Spacer Region Sequence as a Novel Tool for Epizootiological Studies," Avian Diseases 51(2), (1 June 2007). https://doi.org/10.1637/0005-2086(2007)51[555:TMGSRI]2.0.CO;2
Received: 11 October 2006; Accepted: 1 December 2006; Published: 1 June 2007
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top