Improved Culture of Histomonas Meleagridis in a Modification of Dwyer Medium

Harold M. J. F. van der Heijden; Wil J. M. Landman

doi:10.1637/8018-051007-REVIEWR.1

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Harold M. J. F. van der Heijden,^1,* Wil J. M. Landman

¹Animal Health Service (GD), P.O. Box 9, 7400 AA Deventer, the Netherlands

^*Corresponding author. E-mail: h.vd.heijden@gddeventer.com

Abstract

Dwyer medium is the most frequently employed culture medium for Histomonas meleagridis. Both for subculturing and for resuscitation of H. meleagridis from storage in liquid nitrogen, modified Dwyer medium with an increased rice powder concentration (0.8%) and no chicken embryo extract proved superior to Dwyer standard medium, with threefold (10^6.3vs. 10^5.8 histomonads/ml) to 10-fold (10^6.7vs. 10^5.8 histomonads/ml) higher concentrations of parasites after resuscitation or subculturing, respectively.

Citation Download Citation

Harold M. J. F. van der Heijden and Wil J. M. Landman "Improved Culture of Histomonas Meleagridis in a Modification of Dwyer Medium," Avian Diseases 51(4), 986-988, (1 December 2007). https://doi.org/10.1637/8018-051007-REVIEWR.1

Received: 25 May 2007; Accepted: 1 July 2007; Published: 1 December 2007

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