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1 December 2008 Detection of Infectious Bursal Disease Virus Isolates with Unknown Antigenic Properties by Reverse Genetics
Alan H. Icard, Holly S. Sellers, Egbert Mundt
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Abstract

Infectious bursal disease virus (IBDV) serotype 1 is the causative agent of a highly contagious immunosuppressive disease of young chickens. In the past, a number of antigenic, as well as pathogenic, subtypes have been described. The determination of the antigenic makeup of circulating strains is of vital interest to the poultry industry because changes in the antigenicity of circulating field strains have an impact on the use of vaccines. To obtain a more comprehensive overview of the relationship between the nucleotide and amino acid sequence and the antigenic makeup of field isolates, a system based on reverse genetics of IBDV was established. Using this approach, a database for field isolates from three different states in the United States (Georgia, Alabama, and Louisiana), consisting of nucleotide sequence, amino acid sequence, and a reaction pattern based on a panel of monoclonal antibodies, was established. The obtained results showed that phylogenic analysis, which is based on the similarity of sequences, would lead to false conclusions regarding a possible antigenic makeup of the particular isolate. Sequences of field samples were divided into three groups: 1) those that grouped with variant strain E/Del sequences but were antigenically different, 2) those that did not group with sequences of E/Del but were similar in their antigenic makeup, and 3) those that did not group with E/Del sequences and were antigenically different. In addition, using the reverse-genetics approach, a number of field isolates showed no reactivity with any of the used monoclonal antibodies, indicating that an unknown, antigenic subtype of IBDV serotype I is circulating in the field.

Abbreviations: AC-ELISA = antigen-capture enzyme-linked immunosorbent assay; FITC = fluorescein isothiocyanate; IBDV = infectious bursal disease virus; MAb = monoclonal antibodies; NCR = noncoding region; nt = nucleotide; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; QM = quail muscle; RE = restriction endonuclease; RFLP = restriction fragment length polymorphism; RT = reverse transcription; VN = virus neutralization

Detección por medio de genética reversa de aislados de virus de la enfermedad infecciosa de la bolsa con propiedades antigénicas desconocidas.

El serotipo 1 del virus de la enfermedad infecciosa de la bolsa de Fabricio es el agente causal de una enfermedad inmunosupreosora, altamente contagiosa en aves jóvenes. En el pasado se han descrito varios subtipos antigénicos y patogénicos de este virus. La determinación de la composición de cepas circulantes es de vital interés para la industria avícola porque los cambios en la antigenicidad de cepas de campo circulantes tienen un gran impacto en el uso de vacunas. Para obtener un panorama más comprensible de la relación entre las secuencias de nucleótidos y aminoácidos y la composición antigénica de los virus aislados de campo, se estableció un sistema basado en la genética reversa del virus de la enfermedad infecciosa de la bolsa. Usando este enfoque, se estableció una base de datos de los aislados de campo procedentes de tres estados diferentes de los Estados Unidos (Georgia, Alabama y Luisiana), consistente en la secuencia de nucleótidos, secuencia de aminoácidos y el patrón de reacción frente a un panel de anticuerpos monoclonales. Los resultados obtenidos mostraron que el análisis filogenético, basado en la similitud de las secuencias, pueden conducir a conclusiones falsas relacionadas con la composición antigénica de una cepa en particular. Las secuencias de los aislados de campo fueron divididas en tres grupos: 1) Aquellas agrupadas con la secuencia de la cepa variante E/Delaware pero que eran antigénicamente diferentes, 2) Aquellas que no se agruparon en la secuencia de la cepa E/Delaware pero que eran similares en su composición antigénica y 3) Aquellas que no se agruparon con las secuencias de la cep

Alan H. Icard, Holly S. Sellers, and Egbert Mundt "Detection of Infectious Bursal Disease Virus Isolates with Unknown Antigenic Properties by Reverse Genetics," Avian Diseases 52(4), 590-598, (1 December 2008). https://doi.org/10.1637/8302-040408-Reg.1
Received: 7 April 2008; Accepted: 1 July 2008; Published: 1 December 2008
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