Translator Disclaimer
1 March 2010 Pathogenicity of a Quail (Coturnix coturnix japonica)-Derived Marek's Disease Virus Rescued from the QT35 Cell Line
Kelly L. Crucillo, Karel A. Schat, Ynte H. Schukken, Amy E. Brown, Patricia S. Wakenell
Author Affiliations +
Abstract

The QT35 cell line was established in 1977 from methylcholanthrene-induced tumors in Japanese quail. It was later shown that at least some of the QT35 cell lines were latently infected with Marek's disease (MD) virus (MDV). An MDV-like herpesvirus, named quail MDV (QMDV), was isolated from QT35 cells in 2000 by Yamaguchi et al. To determine the pathogenicity of QMDV, we inoculated 10-day-old specific-pathogen-free chickens with QMDV JM (virulent), RB-1B (very virulent), or 584A (very virulent plus). In addition, we inoculated 5-day-old Japanese quail with QMDV, JM, or RB-1B. QMDV is pathogenic in chickens with a tumor incidence comparable to JM. QMDV also caused MD in three out of 18 infected Japanese quail. In conclusion, QMDV is a virulent MDV, and its presence in QT35 cells has implications for the use of QT35 cells for vaccine production.

Kelly L. Crucillo, Karel A. Schat, Ynte H. Schukken, Amy E. Brown, and Patricia S. Wakenell "Pathogenicity of a Quail (Coturnix coturnix japonica)-Derived Marek's Disease Virus Rescued from the QT35 Cell Line," Avian Diseases 54(1), 126-130, (1 March 2010). https://doi.org/10.1637/8722-032309-ResNote.1
Received: 26 March 2009; Accepted: 1 November 2009; Published: 1 March 2010
JOURNAL ARTICLE
5 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top