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1 March 2010 Prokaryotic Recombinant Hemagglutinin-Neuraminidase Protein Enhances the Humoral Response and Efficacy of Commercial Newcastle Disease Vaccines in Chickens
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Abstract

The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays an important role in the pathogenesis of Newcastle disease (ND). Recombinant HN (rHN) protein, produced either by direct injection of recombinant viruses containing HN gene or baculovirus expression systems, has been used to elicit immunity against NDV in chickens. In the present study, a 60.4-kDa rHN was expressed by a prokaryotic expression system and formulated into ND vaccines. Inclusion of rHN (10 µg/ml) into conventional, inactivated ND vaccines significantly (P < 0.05) increased the titer of serum hemagglutination-inhibition Ab in specific-pathogen-free or commercial chickens. Furthermore, when the rHN protein was formulated into ND IC (infectious coryza) bivalent or ND IC FC (fowl cholera) multivalent vaccines, the protection rate of immunized chickens increased from ∼80%–90% to 100% after being challenged by a velogenic strain of NDV. Our data indicated that inclusion of rHN protein produced by an economical prokaryotic expression system could enhance the immunogenicity of traditional and multivalent inactivated ND vaccines. This approach may be adapted to improve the efficacy of ND vaccines currently used in the poultry industry.

Jai-Wei Lee, Ji-Ping Huang, Li-Shian Hong, Shih-Fang Shu, Chi Yu, and Chun-Yen Chu "Prokaryotic Recombinant Hemagglutinin-Neuraminidase Protein Enhances the Humoral Response and Efficacy of Commercial Newcastle Disease Vaccines in Chickens," Avian Diseases 54(1), 53-58, (1 March 2010). https://doi.org/10.1637/8965-062009-Reg.1
Received: 17 July 2009; Accepted: 1 October 2009; Published: 1 March 2010
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