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1 December 2010 Polymerase Chain Reaction-Based Identity Assay for Pathogenic Turkey Eimeria
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Abstract

Diagnosis of turkey Eimeria infection by conventional parasitologic methods is challenging and, until now, no molecular tools existed that clearly distinguished the four widely recognized pathogenic species: Eimeria adenoeides, E. meleagrimitis, E. gallopavonis, and E. dispersa. In this study, the internal transcribed spacer region one (ITS-1) was amplified and sequenced from 23 conventionally characterized reference samples. Phylogenic analysis segregated samples into five distinct cluster groups. The ITS-1 region(s) within each cluster were of a particular length and shared from 96% to 100% identity, while amplified ITS-1 region(s) between clusters differed in length and shared only 10.6% to 49.7% sequence identity. In addition, we developed PCR primer sets as diagnostic tools capable of specifically identifying members of each of the five clusters.

Stephanie M. Cook, Deborrah S. Higuchi, Alexandria L. McGowan, Joan S. Schrader, G. S. K. Withanage, and Michael J. Francis "Polymerase Chain Reaction-Based Identity Assay for Pathogenic Turkey Eimeria," Avian Diseases 54(4), 1152-1156, (1 December 2010). https://doi.org/10.1637/9271-020310-Reg.1
Received: 7 February 2010; Accepted: 1 June 2010; Published: 1 December 2010
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