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1 March 2011 Physical and Genomic Characteristics Identify Chicken Proventricular Necrosis Virus (R11/3 Virus) as a Novel Birnavirus
James S. Guy,, Melissa A. West,, Frederick J Fuller
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Abstract

Chicken proventricular necrosis virus (CPNV), isolate R11/3, previously was isolated from transmissible viral proventriculitis–affected chickens and was determined to be the likely etiology of this disease. CPNV was identified as a birnavirus on the basis of virion size and morphology (icosahedral, approximately 75 nm in diameter, nonenveloped); buoyant density in cesium chloride (1.32 g/ml); a genome comprising bisegmented, double-stranded RNA (approximately 3.8 and 3.4 kilobase pairs); and nucleotide sequence analyses. Nucleotide sequencing of CPNV RNA, segment B, identified a single large open reading frame that encodes a 903–amino acid protein. The 903–amino acid protein was identified as the putative VP1, the viral RNA-dependent RNA polymerase (RdRp), on the basis of sequence homologies with other birnavirus VP1 proteins. The CPNV VP1 possessed the unique permuted RdRp sequence motif arrangement characteristic of birnaviruses; however, phylogenetic analyses based on VP1 demonstrated that CPNV is deeply divergent from other birnaviruses.

American Association of Avian Pathologists
James S. Guy,, Melissa A. West,, and Frederick J Fuller "Physical and Genomic Characteristics Identify Chicken Proventricular Necrosis Virus (R11/3 Virus) as a Novel Birnavirus," Avian Diseases 55(1), 2-7, (1 March 2011). https://doi.org/10.1637/9504-081610-Reg.1
Received: 16 August 2010; Accepted: 1 October 2010; Published: 1 March 2011
JOURNAL ARTICLE
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