Genotyping of Japanese Field Isolates of Mycoplasma synoviae and Rapid Molecular Differentiation from the MS-H Vaccine Strain

Shoji Ogino; Yasuhisa Munakata; Shuichi Ohashi; Masato Fukui; Hiroshi Sakamoto; Yukio Sekiya; Amir H. Noormohammadi; Chris J. Morrow

doi:10.1637/9461-071310-Reg.1

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1 June 2011 Genotyping of Japanese Field Isolates of Mycoplasma synoviae and Rapid Molecular Differentiation from the MS-H Vaccine Strain

Shoji Ogino, Yasuhisa Munakata, Shuichi Ohashi, Masato Fukui, Hiroshi Sakamoto, Yukio Sekiya, Amir H. Noormohammadi, Chris J. Morrow

Author Affiliations +

Shoji Ogino,^1,* Yasuhisa Munakata,¹ Shuichi Ohashi,¹ Masato Fukui,¹ Hiroshi Sakamoto,² Yukio Sekiya,² Amir H. Noormohammadi,³ Chris J. Morrow⁴

¹ACentral Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd., Asaka-machi, Koriyama-shi, Fukushima
²BNippon Biologicals, Inc., Kita Aoyama, Minato-ku, Tokyo 107-0061, Japan
³CSchool of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia
⁴DBioproperties Ltd., Ringwood, Victoria 3134, Australia

^*Corresponding author. E-mail: ogino-shoji@zenoaq.jp

Avian Diseases, 55(2):187-194 (2011). https://doi.org/10.1637/9461-071310-Reg.1

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Abstract

Mycoplasma synoviae is an important causative agent of avian mycoplasmosis. In the present study the conserved domain of the variable lipoprotein and hemagglutinin (vlhA) gene of M. synoviae was sequenced and analyzed for 19 field strains of M. synoviae isolated from chickens across Japan. This analysis revealed that there were at least nine genotypes of M. synoviae present in Japan. Furthermore, we found a single nucleotide polymorphism (SNP) within this region in all the Japanese isolates, and based on this finding, we established a PCR method with cycling probe technology to differentiate between these field isolates and the live M. synoviae vaccine strain Mycoplasma synoviae-H (MS-H).

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Shoji Ogino, Yasuhisa Munakata, Shuichi Ohashi, Masato Fukui, Hiroshi Sakamoto, Yukio Sekiya, Amir H. Noormohammadi, and Chris J. Morrow "Genotyping of Japanese Field Isolates of Mycoplasma synoviae and Rapid Molecular Differentiation from the MS-H Vaccine Strain," Avian Diseases 55(2), 187-194, (1 June 2011). https://doi.org/10.1637/9461-071310-Reg.1

Received: 22 July 2010; Accepted: 1 January 2011; Published: 1 June 2011

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