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1 June 2012 Characterization of Extraintestinal Escherichia coli Isolated from a Peacock (Pavo cristatus) with Colisepticemia
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Abstract

Extraintestinal infections by avian pathogenic strains of Escherichia coli (APEC) are commonly reported in poultry, but there is little information on infections by APEC in other bird species. Here we report on the characterization of extraintestinal E. coli isolated from a domesticated peacock, from the south of Brazil, that died of colisepticemia. Necropsy examination revealed congested liver, hypertrophied kidneys, peritonitis, severe typhlitis suggestive of coligranuloma, pneumonia, and airsacculitis—typical signs of colisepticemia. The isolates from lungs, kidney, heart, intestine, liver, and bone marrow all harbored the same virulence-associated factors (iucD, colV, iss, mat, fimC, ompA, traT crl, csgA vgrG, and hcp), yielded the same band pattern in amplified ribosomal DNA restriction analysis, and were allocated to the Escherichia coli Reference Collection group B1. The isolates were resistant to bacitracin, trimethoprim, and tetracycline, but displayed slight differences in their resistance to other antimicrobials. The isolates also differed in their virulence in 1-day-old chickens, but none displayed high virulence in vivo. We conclude that the peacock died of colisepticemia after it was infected with an extraintestinal E. coli strain of low virulence that nevertheless harbored virulence factors generally associated with APEC. This study represents the first characterization of an APEC isolated from a nonpoultry bird species.

Reporte de Caso—Caracterización de Escherichia coli extra-intestinal aisladas de un pavo real (Pavo cristatus) con colisepticemia.

Las infecciones extraintestinales por cepas patógenas de Escherichia coli aviar (APEC) son frecuentes en la avicultura comercial, sin embargo hay poca información sobre las infecciones por E. coli patógena para las aves en otras especies de aves. En este trabajo, se realizó la caracterización de E. coli extraintestinales aisladas de pavo real domesticado en el sur de Brasil cuya causa de muerte fue colisepticemia. Durante la necropsia del pavo se observó el hígado congestionado, los riñones hipertrofiados, peritonitis, tiflitis severa, lo que sugiere coligranuloma, también se observó neumonía y aerosaculitis, que son lesiones típicas de colisepticemia. Todos los aislamientos de los pulmones, riñones, corazón, intestino, hígado y médula ósea mostraron los mismos factores asociados a la virulencia (iucD, colV, iss, mat, fimC, ompA, traT, crl, csgA, vgrG y hcp), que produjeron el mismo patrón de bandas encontrado mediante el análisis de restricción del ADN ribosomal amplificado, y fueron clasificados dentro del grupo B1 de la Colección de Referencia de Escherichia coli. Los aislamientos fueron resistentes a la bacitracina, a la trimetoprima y a la tetraciclina, pero mostraron ligeras diferencias en su resis

American Association of Avian Pathologists
Nicolle Lima Barbieri, Thiago Moreira Tejkowski, Aline Luísa de Oliveira, Benito Guimarães de Brito, and Fabiana Horn "Characterization of Extraintestinal Escherichia coli Isolated from a Peacock (Pavo cristatus) with Colisepticemia," Avian Diseases 56(2), 436-440, (1 June 2012). https://doi.org/10.1637/9921-090811-Case.1
Received: 13 September 2011; Accepted: 1 January 2012; Published: 1 June 2012