Translator Disclaimer
13 September 2018 Virucidal Efficacy of a Quaternary Ammonium Compound with Food Additive–Grade Calcium Hydroxide Toward Avian Influenza Virus and Newcastle Disease Virus on Abiotic Carriers
Md. Shahin Alam, Satoru Takahashi, Mariko Ito, Miyuki Komura, Mizuki Ono, Chisato Daio, Natthanan Sangsriratanakul, Dany Shoham, Jahangir Alam, Kazuaki Takehara
Author Affiliations +
Abstract

The virucidal efficacies of a 0.2% food additive–grade calcium hydroxide [FdCa(OH)2] solution, a quaternary ammonium compound (QAC) diluted at 1:500 (QACx500), and their mixture [Mix500; FdCa(OH)2 powder added at a final concentration of 0.2% to QACx500] were investigated as fomites for avian influenza virus (AIV) and Newcastle disease virus (NDV) on abiotic carriers (steel, rubber, and plastic) at two different temperatures (room temperature [RT; 25 ± 2 C] and 2 C). These viruses were seeded on coupons (5 cm×5 cm) of rubber, steel, or plastic with 5% fetal bovine serum. After complete drying, the coupons were covered with the test solutions at RT or 2 C. After fixed incubation periods, viruses were recovered from the coupons and titrated. At RT, Mix500 required a short time (3 min) to inactivate AIV and NDV to effective levels (≥3 log virus reduction) on rubber, steel, and plastic carriers compared with QAC or FdCa(OH)2. At low temperature, QACx500 inactivated AIV on steel and plastic carriers to effective levels within 60 min, whereas Mix500 did so within 10 min. QACx500 and FdCa(OH)2 solutions could inactivate NDV on steel and plastic carriers within 20 and 10 min, respectively, and Mix500 could do so within 3 min. Viruses on the carriers required longer incubation periods for inactivation at 2 C than at 25 C. These results demonstrate desirable synergistic virucidal effects of Mix500 for important poultry viruses on abiotic carriers, while indicating high applicability within poultry farming.

Md. Shahin Alam, Satoru Takahashi, Mariko Ito, Miyuki Komura, Mizuki Ono, Chisato Daio, Natthanan Sangsriratanakul, Dany Shoham, Jahangir Alam, and Kazuaki Takehara "Virucidal Efficacy of a Quaternary Ammonium Compound with Food Additive–Grade Calcium Hydroxide Toward Avian Influenza Virus and Newcastle Disease Virus on Abiotic Carriers," Avian Diseases 62(4), 355-363, (13 September 2018). https://doi.org/10.1637/11934-072118-Reg.1
Received: 23 July 2018; Accepted: 11 September 2018; Published: 13 September 2018
JOURNAL ARTICLE
9 PAGES


Share
SHARE
KEYWORDS
abiotic carrier
avian influenza virus
food additive–grade calcium hydroxide
low temperature
Newcastle disease virus
quaternary ammonium compound
synergistic effects
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top