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24 September 2018 Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation
Kazue Nagasawa, Mariko Ishida, Anna Octavera, Kazunari Kusano, Fumi Kezuka, Takeshi Kitano, Yasutoshi Yoshiura, Goro Yoshizaki
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Abstract

A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP).We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (–/–)) recovered their fertility and produced only donorderived fshr (–) X eggs. Natural mating between these females and fshr (–/–) sex-reversed XX males successfully produced large numbers of sterile fshr (–/–) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established.

Summary Sentence

Novel method for mass producing genetically sterile fish from surrogate broodstock was established via transplantation of spermatogonia isolated from follicle-stimulating hormone receptor mutants

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail:journals.permissions@oup.com
Kazue Nagasawa, Mariko Ishida, Anna Octavera, Kazunari Kusano, Fumi Kezuka, Takeshi Kitano, Yasutoshi Yoshiura, and Goro Yoshizaki "Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation," Biology of Reproduction 100(2), 535-546, (24 September 2018). https://doi.org/10.1093/biolre/ioy204
Received: 22 March 2018; Accepted: 21 September 2018; Published: 24 September 2018
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KEYWORDS
fshr mutants
germline stem cells
spermatogonial transplantation
sterile fish production
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