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5 October 2018 Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2
Yue He, Yu Lin, Yu Zhu, Ping Ping, Guishuan Wang, Fei Sun
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Abstract

The expression of many genes during the postmeiotic stages of spermatogenesis is largely regulated by germ cell-specific RNA-binding proteins at the level of posttranscription. One of these RNA-binding proteins, YBX2, participates in mRNA storage and regulation of translation in haploid spermatids. How YBX2-stored mRNAs become translationally competent during spermiogenesis remains unknown. In the present study, we report for the first time that YBX2 interacts with PAIP1, a protein translation enhancer, in vitro and in vivo. In murine testes, PAIP1 is highly expressed and colocalizes with YBX2 in round spermatids. Using sequential RNA immunoprecipitation and sequence analysis, we identified a group of spermiogenic mRNAs indirectly bound by PAIP1 through YBX2. Translation of mRNAs bearing the YBX2 target sequence was significantly blocked by YBX2 protein, but was reinitiated when YBX2 was coexpressed with PAIP1 in vitro. Taken together, these results indicate that PAIP1 regulates the translation of YBX2-masked mRNAs during spermiogenesis, and we propose this YBX2–PAIP1 interaction plays an important role in male germ cell development.

Summary Sentence

PAIP1 interacts with YBX2 in the testis and stimulates translation of spermiogenic mRNAs stored by YBX2.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Yue He, Yu Lin, Yu Zhu, Ping Ping, Guishuan Wang, and Fei Sun "Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2," Biology of Reproduction 100(2), 561-572, (5 October 2018). https://doi.org/10.1093/biolre/ioy213
Received: 14 May 2018; Accepted: 4 October 2018; Published: 5 October 2018
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