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17 October 2019 Proliferation, apoptosis, and number of Sertoli cells in the Syrian hamster during recrudescence after exposure to short photoperiod
Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Concepción Ferrer, María Isabel Serrano-Sánchez, Luis Miguel Pastor
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Abstract

The Sertoli cell (Sc) has been described as a quiescent cell once the animal has reached sexual maturity. Syrian hamster is an animal that displays testicular regression due to short photoperiod, during which process germ cells and Sc are removed through apoptosis. The aim of this work was to investigate histochemically whether the spontaneous testicular recrudescence processes after exposure to a short photoperiod lead to an increase in Sc proliferative activity in order to restore the normal population. Three spontaneous recrudescence groups were established: initial (IR), advanced (AR), and total (TR) recrudescence, which were compared with animal undergoing the regression process (mild: MRg, strong: SRg, and total: TRg) and animals in long photoperiod (Controls). Histological sections were submitted to histochemical techniques for detecting apoptotic and proliferative Sc with bright-field and fluorescence microscopy. For each group, the proliferative Sc index (PScI) and apoptotic Sc index (AScI), and the total number of Sc were obtained. The results revealed the existence of Vimentin+/TUNEL+ as well as Vimentin+/PCNA+ cells. The PScI was significantly higher in TRg and IR than in the other groups. The AScI was only significantly higher in MRg and SRg with respect to the other groups. The total number of Sc increased among TRg, IR, and AR, reaching values similar to those of the Controls. In conclusion, the increase in Sc proliferation from final regression and recrudescence, accompanied by a similar rate of apoptosis to the Control group, is the cause of the restoration of the Sc population during spontaneous recrudescence.

Summary Sentence

The total population of Scs is recovered after spontaneous recrudescence due to an increase in Sc proliferation.

© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Concepción Ferrer, María Isabel Serrano-Sánchez, and Luis Miguel Pastor "Proliferation, apoptosis, and number of Sertoli cells in the Syrian hamster during recrudescence after exposure to short photoperiod," Biology of Reproduction 102(3), 588-597, (17 October 2019). https://doi.org/10.1093/biolre/ioz198
Received: 27 December 2018; Accepted: 7 October 2019; Published: 17 October 2019
KEYWORDS
Apoptosis
proliferation
quiescence
recrudescence
Sertoli cell numbers
short photoperiod
Syrian hamster
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