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9 September 2021 Exposure to nivalenol declines mouse oocyte quality via inducing oxidative stress-related apoptosis and DNA damage
Yue Wang, Chun-Hua Xing, Hao-Lin Zhang, Zhen-Nan Pan, Shao-Chen Su
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Abstract

Mammalian oocyte quality is critical for fertilization and early embryo development. The type B trichothecene nivalenol (NIV) is a mycotoxin produced by Fusarium oxysporum, and it is commonly found with deoxynivalenol in contaminated food or feed. NIV has been shown to affect the immune system and female reproductive system, cause emesis and growth retardation. Here, we investigated the toxicity of NIV on mouse oocyte quality, as well as the protective effects of melatonin on the NIV-exposed oocytes. We found NIV exposure caused meiotic arrest and further induced the failure of polar body extrusion in mouse oocytes. Transcriptome analysis data showed that NIV exposure altered the expression of multiple pathway-related genes in oocytes, indicating its wide toxicity on oocyte maturation. Based on the RNA-seq data, we showed that NIV exposure induced oxidative stress and caused DNA damage in oocytes. Besides, autophagy, and early apoptosis were also found in NIV-exposed oocytes. Treatment with melatonin significantly ameliorated these defects through its effects on ROS level. Thus, our results demonstrated that exposure to NIV affected oocyte quality and melatonin treatment could reduce the defects caused by NIV in mouse oocytes.

© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions please e-mail: journals.permissions@oup.com
Yue Wang, Chun-Hua Xing, Hao-Lin Zhang, Zhen-Nan Pan, and Shao-Chen Su "Exposure to nivalenol declines mouse oocyte quality via inducing oxidative stress-related apoptosis and DNA damage," Biology of Reproduction 105(6), 1474-1483, (9 September 2021). https://doi.org/10.1093/biolre/ioab171
Received: 16 June 2021; Accepted: 7 September 2021; Published: 9 September 2021
KEYWORDS
meiosis
Melatonin
nivalenol
oocyte maturation
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