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13 December 2021 ZDHHC19 localizes to the cell membrane of spermatids and is involved in spermatogenesis
Yangyang Wu, Xin Zhang, Xi Zhang, Siyu Liu, Jintao Zhang, Shuya Sun, Shuqin Zhao, Zerui Wang, Yiqiang Cui, Xiaoyan Huang, Mingxi Liu
Author Affiliations +
Abstract

Sperm is the ultimate executor of male reproductive function. Normal morphology, quantity, and motility of sperm ensure the normal reproductive process. Palmitoylation is a posttranslational modification mediated by palmitoyltransferases whereby palmitoyl is added to proteins. Seven palmitoyltransferases have been identified in Saccharomyces cerevisiae and 23 in humans (including ZDHHC1–9 and ZDHHC11–24), with corresponding homologs in mice. We identified two testis-specific palmitoyltransferases ZDHHC11 and ZDHHC19 in mice. The Zdhhc11 and Zdhhc19-knockout mouse models were constructed, and it was found that the Zdhhc11 knockout males were fertile, while Zdhhc19 knockout males were sterile. ZDHHC19 is located in the cell membrane of step 4–9 spermatids in the mouse testis, and phenotypic analysis showed that the testicular weight ratio in the Zdhhc19-/- mice decreased along with the number and motility of the sperm decreased, while sperm abnormalities increased, mainly due to the “folded” abnormal sperm caused by sperm membrane fusion, suggesting the involvement of ZDHHC19 in maintaining membrane stability in the male reproductive system. In addition, Zdhhc19-/- mice showed abnormal sperm morphologies and apoptosis during spermatogenesis, suggesting that spermatogenesis in the Zdhhc19-/- mice was abnormal. These results indicate that ZDHHC19 promotes membrane stability in male germ cells.

Summary Sentence

ZDHHC19 is located in the cell membrane of Step4–9 spermatids in mouse testis; Zdhhc19 knockout mice showed male infertility, abnormal spermatogenesis, sperm morphology and motility.

© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Yangyang Wu, Xin Zhang, Xi Zhang, Siyu Liu, Jintao Zhang, Shuya Sun, Shuqin Zhao, Zerui Wang, Yiqiang Cui, Xiaoyan Huang, and Mingxi Liu "ZDHHC19 localizes to the cell membrane of spermatids and is involved in spermatogenesis," Biology of Reproduction 106(3), 477-486, (13 December 2021). https://doi.org/10.1093/biolre/ioab224
Received: 27 August 2021; Accepted: 8 December 2021; Published: 13 December 2021
KEYWORDS
Male infertility
palmitoylation
teratospermia
ZDHHC11
ZDHHC19
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