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25 May 2022 Multiomics analysis of male infertility
Xiaolong Wu, Liwei Zhou, Jie Shi, C. Yan Cheng, Fei Sun
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Abstract

Infertility affects 8–12% of couples globally, and the male factor is a primary cause in ∼50% of couples. Male infertility is a multifactorial reproductive disorder, which can be caused by paracrine and autocrine factors, hormones, genes, and epigenetic changes. Recent studies in rodents and most notably in humans using multiomics approach have yielded important insights into understanding the biology of spermatogenesis. Nonetheless, the etiology and pathogenesis of male infertility are still largely unknown. In this review, we summarized and critically evaluated findings based on the use of advanced technologies to compare normal and obstructive azoospermic versus nonobstructive azoospermic men, including whole-genome bisulfite sequencing, single-cell RNA-seq, whole-exome sequencing, and transposase-accessible chromatin using sequencing. It is obvious that the multiomics approach is the method of choice for basic research and clinical studies including clinical diagnosis of male infertility.

Summary Sentence

This review summarizes findings of the past 20 years published in literature listed in PubMed including our laboratory, utilizing multiomics approach to study the etiology and pathogenesis of male infertility.

Graphical Abstract

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© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Xiaolong Wu, Liwei Zhou, Jie Shi, C. Yan Cheng, and Fei Sun "Multiomics analysis of male infertility," Biology of Reproduction 107(1), 118-134, (25 May 2022). https://doi.org/10.1093/biolre/ioac109
Received: 13 December 2021; Accepted: 17 May 2022; Published: 25 May 2022
JOURNAL ARTICLE
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KEYWORDS
human infertility
multiomics
scATAC-seq
single-cell RNA-seq (scRNA-seq)
spermatogenesis
whole-exome sequencing (WES)
whole-genome bisulfite sequencing (WGBS)
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