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1 June 2000 Detection of Progesterone Receptor Transcript in Human Spermatozoa
Geetanjali Sachdeva, Chirag Ashwinkumar Shah, Sanjiva Dattatraya Kholkute, Chander Prakash Puri
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Abstract

The present study, to our knowledge, is the first to demonstrate presence of progesterone receptor (PR) transcript in human spermatozoa. The study shows the presence of low copy number PR mRNA in mature human spermatozoa. The PR transcript in spermatozoa was detected by reverse transcriptase-polymerase chain reaction using primers specific for the hormone binding domain and the DNA binding domain of the conventional uterine PR. Further, the cDNA sequence of the partial PR transcript from spermatozoa was found to be identical to the region spanning nucleotides 2694 to 3230 of the conventional PR full-length cDNA sequence. This study also indirectly suggests that the PR protein indeed is an intrinsic sperm protein and is not acquired through proteinaceous secretions of accessory reproductive organs.

Geetanjali Sachdeva, Chirag Ashwinkumar Shah, Sanjiva Dattatraya Kholkute, and Chander Prakash Puri "Detection of Progesterone Receptor Transcript in Human Spermatozoa," Biology of Reproduction 62(6), 1610-1614, (1 June 2000). https://doi.org/10.1095/biolreprod62.6.1610
Received: 1 June 1999; Accepted: 1 January 2000; Published: 1 June 2000
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