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1 June 2000 Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release, and Parthenogenetic Development in Bovine Oocytes
Kenneth D. Campbell, William A. Reed, Kenneth L. White
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Abstract

The ability of arginine-glycine-aspartic acid (RGD; a sequence recognized by integrins) or non-RGD-containing peptides to block fertilization, induce intracellular Ca2 oscillations, and initiate parthenogenetic development in bovine oocytes was investigated. Addition of a soluble RGD peptide during fertilization at concentrations ranging from 10 to 1000 μg/ml significantly decreased (P < 0.05) fertilization as compared to the in vitro-fertilized controls. The addition of non-RGD peptide had no effect on fertilization. Two intracellular Ca2 transients 21.5 ± 1.9 min apart were observed in 56 of 60 oocytes incubated in RGD peptide concentrations ranging from 20 to 1000 μg/ml. No intracellular Ca2 transients were observed in medium alone, non-RGD treatment groups or in the RGD peptide at 10 μg/ml. The percentage of oocytes activated with ionomycin and 6-dimethylaminopurine (63% cleavage and 34% blastocyst development) was significantly higher (P < 0.05) than those activated with the RGD peptide and 6-dimethylaminopurine (35% cleavage and 19% blastocyst development). These groups were significantly higher (P < 0.05) than either peptide alone, 6-dimethylaminopurine alone, or the non-RGD peptide and 6-dimethylaminopurine treatment groups. These data provide evidence that ligation of an integrin on bovine oocytes with a soluble RGD peptide is capable of blocking fertilization, inducing intracellular Ca2 transients, and initiating parthenogenetic development.

Kenneth D. Campbell, William A. Reed, and Kenneth L. White "Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release, and Parthenogenetic Development in Bovine Oocytes," Biology of Reproduction 62(6), 1702-1709, (1 June 2000). https://doi.org/10.1095/biolreprod62.6.1702
Received: 10 December 1999; Accepted: 1 February 2000; Published: 1 June 2000
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