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1 August 2000 Administration of Prostaglandin F During the Early Bovine Luteal Phase Does Not Alter the Expression of ET-1 and of Its Type A Receptor: A Possible Cause for Corpus Luteum Refractoriness
Nitzan Levy, Shu-ichi Kobayashi, Zvi Roth, David Wolfenson, Akio Miyamoto, Rina Meidan
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Abstract

Luteal regression is initiated by prostaglandin F (PGF). In domestic species and primates, demise of the corpus luteum (CL) enables development of a new preovulatory follicle. However, during early stages of the cycle, which are characterized by massive neovascularization, the CL is refractory to PGF. Our previous studies showed that endothelin-1 (ET-1), which is produced by the endothelial cells lining these blood vessels, plays a crucial role during PGF-induced luteolysis. Therefore, in this study, we compared the effects of PGF administered at the early and mid luteal phases on ET-1 and its type A receptors (ETA-R) along with plasma ET-1 and progesterone concentrations, and the mRNA levels of PGF receptors (PGF-R) and steroidogenic genes. As expected, ET-1 and ETA-R mRNA levels were markedly induced in midcycle CL exposed to luteolytic dose of PGF analogue (Cloprostenol). In contrast, neither ET-1 mRNA nor its receptors were elevated when the same dose of PGF analogue was administered on Day 4 of the cycle. In accordance with ET-1 expression within the CL, plasma ET-1 concentrations were significantly elevated 24 h after PGF injection only on Day 10 of the cycle. The steroidogenic capacity of the CL (plasma progesterone as well as the mRNA levels of steroidogenic acute regulatory protein and cytochrome P450scc) was only affected when PGF was administered during midcycle. Nevertheless, PGF elicited certain responses in the early CL: progesterone and oxytocin secretion were elevated, and PGF-R was transiently affected. Such effects probably result from PGF acting on luteal steroidogenic cells. These findings may suggest, however, that the cell type mediating the luteolytic actions of PGF, possibly the endothelium, could yet be nonresponsive during the early luteal phase.

Nitzan Levy, Shu-ichi Kobayashi, Zvi Roth, David Wolfenson, Akio Miyamoto, and Rina Meidan "Administration of Prostaglandin F During the Early Bovine Luteal Phase Does Not Alter the Expression of ET-1 and of Its Type A Receptor: A Possible Cause for Corpus Luteum Refractoriness," Biology of Reproduction 63(2), 377-382, (1 August 2000). https://doi.org/10.1095/biolreprod63.2.377
Received: 11 October 1999; Accepted: 1 March 2000; Published: 1 August 2000
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