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1 August 2000 Interferon-τ Modulates Phorbol Ester-Induced Production of Prostaglandin and Expression of Cyclooxygenase-2 and Phospholipase-A2 from Bovine Endometrial Cells
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Abstract

Antiluteolytic actions of bovine interferon-tau (bIFN-τ) require suppression of prostaglandin F (PGF) production. Our objective was to test whether bIFN-τ could block PGF production and synthesis of phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) enzymes induced by a protein kinase C (PKC) stimulator (phorbol 12,13 dibutyrate; PDBu). Bovine endometrial epithelial (BEND) cells were treated with PDBu in the presence or absence of bIFN-τ. Medium samples were analyzed for concentrations of PGF, whole-cell extracts were analyzed for abundance of PLA2 and COX-2 by immunoblotting, and RNA extracts were examined for steady-state levels of COX-2 mRNA by Northern blotting. The PDBu stimulated production of PGF between 3 and 12 h, levels of COX-2 mRNA by 3 h and protein expression of COX-2 and PLA2 by 6 and 12 h, respectively. Added concomitantly with PDBu, bIFN-τ suppressed PGF production, steady-state levels of COX-2 mRNA, and expression of COX-2 and PLA2 proteins. Added after a 3-h stimulation with PDBu alone, bIFN-τ suppressed PGF production after 1 h. Bovine IFN-τ inhibited intracellular mechanisms responsible for PGF production in BEND cells, and this could be through both cytosolic and nuclear actions.

Mario Binelli, Aydin Guzeloglu, Lokenga Badinga, Daniel R. Arnold, Jean Sirois, Thomas R. Hansen, and William W. Thatcher "Interferon-τ Modulates Phorbol Ester-Induced Production of Prostaglandin and Expression of Cyclooxygenase-2 and Phospholipase-A2 from Bovine Endometrial Cells," Biology of Reproduction 63(2), 417-424, (1 August 2000). https://doi.org/10.1095/biolreprod63.2.417
Received: 31 December 1999; Accepted: 1 March 2000; Published: 1 August 2000
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