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1 October 2000 Chromosome Aberrations in In Vitro-Produced Bovine Embryos at Days 2–5 Post-Insemination
Dorthe Viuff, Torben Greve, Birthe Avery, Poul Hyttel, Per B. Brockhoff, Preben D. Thomsen
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Abstract

Availability of embryos of high quality is required to obtain satisfactory embryonic developmental rates and normal calves following transfer of in vitro-produced (IVP) bovine embryos. One relevant quality parameter is the frequency of chromosome aberrations, which can be evaluated using multicolor fluorescent in situ hybridization (FISH) with chromosome 6- and chromosome 7-specific probes in cattle. In this study, interphase nuclei (n = 3805) were analyzed from 426 bovine IVP embryos. We found that 73%, 72%, 81%, and 58% of the embryos from Days 2, 3, 4, and 5 post-insemination (pi), respectively, displayed a normal diploid chromosome number in all cells. When looking at the types of chromosome aberrations, the percentages of mixoploidy at Days 2, 3, 4, and 5 pi were 22%, 15%, 16%, and 42%, respectively, whereas the percentages of polyploidy (i.e., all nuclei in an embryo were analyzed and were polyploid) were 5%, 13%, 3%, and 0%, respectively. In conclusion, numerical chromosome aberrations were detected as early as Day 2 pi. The development of polyploid embryos is slow and is apparently arrested during the third cell cycle, whereas the mixoploid embryos seem to continue development.

Dorthe Viuff, Torben Greve, Birthe Avery, Poul Hyttel, Per B. Brockhoff, and Preben D. Thomsen "Chromosome Aberrations in In Vitro-Produced Bovine Embryos at Days 2–5 Post-Insemination," Biology of Reproduction 63(4), 1143-1148, (1 October 2000). https://doi.org/10.1095/biolreprod63.4.1143
Received: 15 February 2000; Accepted: 1 May 2000; Published: 1 October 2000
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