Translator Disclaimer
1 December 2000 Effects of Sodium Pyruvate in Nonserum Maturation Medium on Maturation, Fertilization, and Subsequent Development of Bovine Oocytes With or Without Cumulus Cells
Author Affiliations +
Abstract

The present study was conducted to determine the effects of cumulus cells and sodium pyruvate during in vitro maturation of bovine oocytes on maturation, fertilization, and subsequent development. Cumulus-enclosed oocytes (CEOs) and cumulus-denuded oocytes (CDOs) were cultured for 24 h in polyvinylpyrrolidone-Hepes-tissue culture medium 199 with or without sodium pyruvate. Oocytes were fertilized in vitro and then cultured in CR1aa for 10 days. Before in vitro fertilization, the glutathione (GSH) content of some oocytes was measured. Maturation and normal fertilization rates of CDOs cultured with sodium pyruvate and CEOs were higher than that of CDOs cultured without sodium pyruvate. The CEOs showed significantly higher rates of development to the blastocyst stage than CDOs. The GSH contents of oocytes significantly decreased in CDOs after maturation culture, but the GSH contents of oocytes in CEOs remained at the same level as oocytes before culture. These results indicate that sodium pyruvate promotes nuclear maturation of bovine CDOs and that a continuing presence of cumulus cells during maturation is important for subsequent development of zygotes to the blastocyst stage. However, blastocysts produced from CDOs in the presence of sodium pyruvate showed a developmental competence to be normal calves, but it is not known if CDOs cultured without sodium pyruvate also were capable of developing into calves.

Masaya Geshi, Naoki Takenouchi, Nobuhiko Yamauchi, and Takashi Nagai "Effects of Sodium Pyruvate in Nonserum Maturation Medium on Maturation, Fertilization, and Subsequent Development of Bovine Oocytes With or Without Cumulus Cells," Biology of Reproduction 63(6), 1730-1734, (1 December 2000). https://doi.org/10.1095/biolreprod63.6.1730
Received: 22 March 2000; Accepted: 1 July 2000; Published: 1 December 2000
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top