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1 December 2000 Effect of Prolactin and Androgen on the Expression of the Female-Attracting Pheromone Silefrin in the Abdominal Gland of the Newt, Cynops ensicauda
Takeo Iwata, Genri Kawahara, Kazutoshi Yamamoto, Cheng Ji Zhou, Shigeo Nakajo, Seiji Shioda, Sakaé Kikuyama
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Abstract

Silefrin is a sodefrin-like, female-attracting pheromone comprising 10 amino acids that was isolated from the abdominal gland of the sword-tailed newt, Cynops ensicauda. Hormonal effects on the silefrin precursor mRNA expression and silefrin content in the abdominal gland were investigated in the present study by using Northern blot analysis and radioimmunoassay, respectively. In the abdominal gland of newts treated with prolactin (PRL) plus testosterone propionate (TP), silefrin precursor mRNA expression was markedly enhanced as compared with that in the newts injected with saline, PRL, or TP. Values for radioimmunoassayable silefrin content in the abdominal gland paralleled those for the silefrin precursor mRNA levels. Moreover, silefrin precursor mRNA signals, as revealed by in situ hybridization, as well as stainability of immunoreactive silefrin were much more intense in the epithelial cells of the abdominal gland of the PRL-plus-TP-treated animals than in those of controls. We thus conclude that PRL and androgen are important factors for enhancing silefrin synthesis.

Takeo Iwata, Genri Kawahara, Kazutoshi Yamamoto, Cheng Ji Zhou, Shigeo Nakajo, Seiji Shioda, and Sakaé Kikuyama "Effect of Prolactin and Androgen on the Expression of the Female-Attracting Pheromone Silefrin in the Abdominal Gland of the Newt, Cynops ensicauda," Biology of Reproduction 63(6), 1867-1872, (1 December 2000). https://doi.org/10.1095/biolreprod63.6.1867
Received: 1 May 2000; Accepted: 1 August 2000; Published: 1 December 2000
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KEYWORDS
pheromones
prolactin
reproductive behavior
testosterone
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