We examined the in vitro developmental potential of nonactivated and activated enucleated ova receiving cumulus cells at various stages of the cell cycle. Eleven to 29% of activated ova receiving donor cells stopped developing at the 8-cell stage but 21% to 50% of nonactivated ova receiving donor cells at either the G0, G1, G2, or M phase, or cycling cells developed into blastocysts. One normal calf was born after transferring five blastocysts that had developed from ova receiving donor cells at the M phase. The present study demonstrated that direct exposure of donor chromosomes to nonactivated ovum cytoplasm is effective for somatic cell nucleus reprogramming, and activated ovum cytoplasm does not reprogram the nucleus.
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1 January 2001
Direct Exposure of Chromosomes to Nonactivated Ovum Cytoplasm Is Effective for Bovine Somatic Cell Nucleus Reprogramming
Tetsuya Tani,
Yoko Kato,
Yukio Tsunoda
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developmental biology
implantation/early development