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1 February 2001 Bovine Interferon-τ Stimulates the Janus Kinase-Signal Transducer and Activator of Transcription Pathway in Bovine Endometrial Epithelial Cells
Mario Binelli, Prem Subramaniam, Thais Diaz, Greg A. Johnson, Thomas R. Hansen, Lokenga Badinga, William W. Thatcher
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Abstract

Trophoblastic bovine interferon-tau (bIFN-τ) suppresses luteolytic pulses of endometrial prostaglandin F (PGF) at the time of maternal recognition of pregnancy. This results in maintenance of the corpus luteum in cattle. The hypothesis that effects of bIFN-τ in the endometrium were through activation of the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway of signal transduction was tested. Whole cell, cytosolic, and nuclear extracts from bovine endometrial cells treated with bIFN-τ were analyzed by immunoprecipitation, immunoblotting, and electrophoretic mobility shift assays in a series of dose- and time-dependency experiments. Bovine IFN-τ stimulated tyrosine phosphorylation, homo- and heterodimer formation, nuclear translocation, and DNA binding of STAT proteins 1, 2, and 3. Moreover, bIFN-τ induced synthesis of interferon-regulatory factor. In conclusion, bIFN-τ stimulates the JAK-STAT pathway in the bovine endometrium. It is proposed that activation of the JAK-STAT pathway is involved in regulating the antiluteolytic effects of bIFN-τ.

Mario Binelli, Prem Subramaniam, Thais Diaz, Greg A. Johnson, Thomas R. Hansen, Lokenga Badinga, and William W. Thatcher "Bovine Interferon-τ Stimulates the Janus Kinase-Signal Transducer and Activator of Transcription Pathway in Bovine Endometrial Epithelial Cells," Biology of Reproduction 64(2), 654-665, (1 February 2001). https://doi.org/10.1095/biolreprod64.2.654
Received: 17 May 2000; Accepted: 1 October 2000; Published: 1 February 2001
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