Translator Disclaimer
1 May 2001 Aromatase Messenger RNA Is Derived from the Proximal Promoter of the Aromatase Gene in Leydig, Sertoli, and Germ Cells of the Rat Testis
Marilena Lanzino, Stefania Catalano, Christelle Genissel, Sebastiano Ando, Serge Carreau, Kent Hamra, Michael J. McPhaul
Author Affiliations +
Abstract

It has long been recognized that individual cell types within the testes possess the capacity to synthesize estrogen. A number of studies on different species have demonstrated that the levels of aromatase expression and the patterns of regulation are distinct between the different cell types of the testes. Whereas a variety of promoters have been shown to contribute to the patterns of aromatase expression in different cell lineages, studies using ovarian RNA, testis RNA, and Leydig cell tumor lines have demonstrated that the same promoter (promoter II) was used in each. Recent experiments using potent aromatase inhibitors or analysis of animals in which the genes encoding the estrogen receptor-alpha (ER-α) or the aromatase, P450, are defective, have confirmed the importance of local estrogen formation in normal testicular function. In order to permit experiments to identify the elements controlling aromatase expression in the individual cell compartments of the testes, we prepared RNA from purified preparations of Leydig, Sertoli, and germ cells. Using specific oligonucleotide primers, the sites of initiation of the aromatase mRNA were determined using rapid amplification of cDNA ends (RACE) and nucleotide sequence analysis of the resulting cDNA fragments. Our results indicate that aromatase mRNA is derived from the proximal promoter (PII) of the aromatase gene in each of the major cell types of the rat testes.

Marilena Lanzino, Stefania Catalano, Christelle Genissel, Sebastiano Ando, Serge Carreau, Kent Hamra, and Michael J. McPhaul "Aromatase Messenger RNA Is Derived from the Proximal Promoter of the Aromatase Gene in Leydig, Sertoli, and Germ Cells of the Rat Testis," Biology of Reproduction 64(5), 1439-1443, (1 May 2001). https://doi.org/10.1095/biolreprod64.5.1439
Received: 14 September 2000; Accepted: 1 December 2000; Published: 1 May 2001
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top