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1 July 2001 Chromosomal Abnormalities and Developmental Kinetics in In Vivo-Developed Cattle Embryos at Days 2 to 5 after Ovulation
Dorthe Viuff, Peter J. M. Hendriksen, Peter L. A. M. Vos, Steph J. Dieleman, Bo M. Bibby, Torben Greve, Poul Hyttel, Preben D. Thomsen
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Abstract

The frequency of chromosome abnormalities was investigated in cattle embryos (n = 256) derived from superovulated heifers (n = 35) on Days 2, 3, 4, and 5 postovulation (PO). Interphase nuclei (n = 4358) were analyzed for chromosome abnormalities using fluorescent in situ hybridization with chromosome 6- and chromosome 7-specific probes and the developmental rate was described by scoring cell numbers. We found that 93%, 85%, 84%, and 69% of the embryos from Days 2, 3, 4, and 5 PO, respectively, displayed a normal diploid chromosome number in all cells. Of the embryos containing abnormal cells, mixoploidy was significantly more frequent than polyploidy. The percentage of mixoploidy at Days 2, 3, 4, and 5 PO was 5%, 13%, 16%, and 31%, respectively, whereas the percentages of polyploidy were 2%, 2%, 0%, and 0%, respectively. The mean number of cells per embryo was 4.7, 8, 11.5, and 48.3, respectively, at Days 2, 3, 4, and 5 PO. Thus, in vivo-developed embryos were significantly more advanced than the in vitro-produced (IVP) embryos except for Day 2. In conclusion, a significantly lower frequency of chromosomally abnormal embryos, in particular displaying polyploidy early after fertilization, was seen in in vivo versus IVP embryos, and these chromosomal abnormalities may be inherent to the process of IVP in cattle.

Dorthe Viuff, Peter J. M. Hendriksen, Peter L. A. M. Vos, Steph J. Dieleman, Bo M. Bibby, Torben Greve, Poul Hyttel, and Preben D. Thomsen "Chromosomal Abnormalities and Developmental Kinetics in In Vivo-Developed Cattle Embryos at Days 2 to 5 after Ovulation," Biology of Reproduction 65(1), 204-208, (1 July 2001). https://doi.org/10.1095/biolreprod65.1.204
Received: 10 January 2001; Accepted: 1 February 2001; Published: 1 July 2001
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KEYWORDS
conceptus
developmental biology
implantation/early development
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